Gibberellic acid induces α-amylase expression in adipose-derived stem cells.
Identifieur interne : 000285 ( Main/Exploration ); précédent : 000284; suivant : 000286Gibberellic acid induces α-amylase expression in adipose-derived stem cells.
Auteurs : Atsushi Kasamatsu [Japon] ; Manabu Iyoda ; Katsuya Usukura ; Yosuke Sakamoto ; Katsunori Ogawara ; Masashi Shiiba ; Hideki Tanzawa ; Katsuhiro UzawaSource :
- International journal of molecular medicine [ 1791-244X ] ; 2012.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Cellules cultivées (MeSH), Cellules souches (cytologie), Cellules souches (effets des médicaments et des substances chimiques), Cellules souches (métabolisme), Différenciation cellulaire (effets des médicaments et des substances chimiques), Expression des gènes (MeSH), Gibbérellines (pharmacologie), Gibbérellines (toxicité), Humains (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Tissu adipeux (cytologie), Tissu adipeux (métabolisme), alpha-Amylases (génétique), alpha-Amylases (métabolisme).
- MESH :
- cytologie : Cellules souches, Tissu adipeux.
- effets des médicaments et des substances chimiques : Cellules souches, Différenciation cellulaire, Survie cellulaire.
- génétique : alpha-Amylases.
- métabolisme : ARN messager, Cellules souches, Tissu adipeux, alpha-Amylases.
- pharmacologie : Gibbérellines.
- toxicité : Gibbérellines.
- Cellules cultivées, Expression des gènes, Humains.
English descriptors
- KwdEn :
- Adipose Tissue (cytology), Adipose Tissue (metabolism), Cell Differentiation (drug effects), Cell Survival (drug effects), Cells, Cultured (MeSH), Gene Expression (MeSH), Gibberellins (pharmacology), Gibberellins (toxicity), Humans (MeSH), RNA, Messenger (metabolism), Stem Cells (cytology), Stem Cells (drug effects), Stem Cells (metabolism), alpha-Amylases (genetics), alpha-Amylases (metabolism).
- MESH :
- chemical , genetics : alpha-Amylases.
- chemical , metabolism : RNA, Messenger, alpha-Amylases.
- chemical , pharmacology : Gibberellins.
- cytology : Adipose Tissue, Stem Cells.
- drug effects : Cell Differentiation, Cell Survival, Stem Cells.
- metabolism : Adipose Tissue, Stem Cells.
- chemical , toxicity : Gibberellins.
- Cells, Cultured, Gene Expression, Humans.
Abstract
Salivary α-amylase is the most important enzyme for oral digestion of dietary starch. Therefore, regeneration of the salivary glands via a tissue engineering approach is clearly required for patients with salivary gland dysfunction. Early during seed germination, the embryo synthesizes gibberellic acid (GA3), a plant hormone that activates the synthesis and secretion of α-amylase. The purpose of this study was to explore an approach for differentiation of stem cells into salivary glands using GA3. We isolated adipose-derived stem cells (ASCs), which are positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and possess pluripotency to osteoblasts, adipocytes and neural cells, from human buccal fat pads, which are a readily available source for dentists and oral surgeons. In addition, we investigated the cytotoxicity of GA3 for human ASCs. GA3 neither affects cell morphology nor cell viability in a dose- or time-dependent manner. ASCs were incubated with GA3 to assess mRNA and protein expression of α-amylase by reverse transcriptase-polymerase chain reaction and western blot analyses. α-amylase mRNA expression on 21 days after treatment with GA3 (1 mM) was seven times greater than that in resting condition (Day 0). While we did not detect α-amylase bands on Day 0, α-amylase protein was detectable 7 days after treatment with GA3, reaching a maximal level on Day 21. Our results indicated that GA3 can increase cellular α-amylase expression and that our induction method would be useful for therapeutic application for salivary gland regeneration.
DOI: 10.3892/ijmm.2012.1007
PubMed: 22641429
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Usukura">Katsuya Usukura</name></author><author><name sortKey="Sakamoto, Yosuke" sort="Sakamoto, Yosuke" uniqKey="Sakamoto Y" first="Yosuke" last="Sakamoto">Yosuke Sakamoto</name></author><author><name sortKey="Ogawara, Katsunori" sort="Ogawara, Katsunori" uniqKey="Ogawara K" first="Katsunori" last="Ogawara">Katsunori Ogawara</name></author><author><name sortKey="Shiiba, Masashi" sort="Shiiba, Masashi" uniqKey="Shiiba M" first="Masashi" last="Shiiba">Masashi Shiiba</name></author><author><name sortKey="Tanzawa, Hideki" sort="Tanzawa, Hideki" uniqKey="Tanzawa H" first="Hideki" last="Tanzawa">Hideki Tanzawa</name></author><author><name sortKey="Uzawa, Katsuhiro" sort="Uzawa, Katsuhiro" uniqKey="Uzawa K" first="Katsuhiro" last="Uzawa">Katsuhiro Uzawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22641429</idno><idno type="pmid">22641429</idno><idno type="doi">10.3892/ijmm.2012.1007</idno><idno 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260-8670</wicri:regionArea><wicri:noRegion>Chiba 260-8670</wicri:noRegion></affiliation></author><author><name sortKey="Iyoda, Manabu" sort="Iyoda, Manabu" uniqKey="Iyoda M" first="Manabu" last="Iyoda">Manabu Iyoda</name></author><author><name sortKey="Usukura, Katsuya" sort="Usukura, Katsuya" uniqKey="Usukura K" first="Katsuya" last="Usukura">Katsuya Usukura</name></author><author><name sortKey="Sakamoto, Yosuke" sort="Sakamoto, Yosuke" uniqKey="Sakamoto Y" first="Yosuke" last="Sakamoto">Yosuke Sakamoto</name></author><author><name sortKey="Ogawara, Katsunori" sort="Ogawara, Katsunori" uniqKey="Ogawara K" first="Katsunori" last="Ogawara">Katsunori Ogawara</name></author><author><name sortKey="Shiiba, Masashi" sort="Shiiba, Masashi" uniqKey="Shiiba M" first="Masashi" last="Shiiba">Masashi Shiiba</name></author><author><name sortKey="Tanzawa, Hideki" sort="Tanzawa, Hideki" uniqKey="Tanzawa H" first="Hideki" last="Tanzawa">Hideki Tanzawa</name></author><author><name sortKey="Uzawa, Katsuhiro" sort="Uzawa, Katsuhiro" uniqKey="Uzawa K" first="Katsuhiro" last="Uzawa">Katsuhiro Uzawa</name></author></analytic><series><title level="j">International journal of molecular medicine</title><idno type="eISSN">1791-244X</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adipose Tissue (cytology)</term><term>Adipose Tissue (metabolism)</term><term>Cell Differentiation (drug effects)</term><term>Cell Survival (drug effects)</term><term>Cells, Cultured (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gibberellins (pharmacology)</term><term>Gibberellins (toxicity)</term><term>Humans (MeSH)</term><term>RNA, Messenger (metabolism)</term><term>Stem Cells (cytology)</term><term>Stem Cells (drug effects)</term><term>Stem Cells (metabolism)</term><term>alpha-Amylases (genetics)</term><term>alpha-Amylases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Cellules souches (cytologie)</term><term>Cellules souches (effets des médicaments et des substances chimiques)</term><term>Cellules souches (métabolisme)</term><term>Différenciation cellulaire (effets des médicaments et des substances chimiques)</term><term>Expression des gènes (MeSH)</term><term>Gibbérellines (pharmacologie)</term><term>Gibbérellines (toxicité)</term><term>Humains (MeSH)</term><term>Survie cellulaire (effets des médicaments et des substances chimiques)</term><term>Tissu adipeux (cytologie)</term><term>Tissu adipeux (métabolisme)</term><term>alpha-Amylases (génétique)</term><term>alpha-Amylases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>alpha-Amylases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term><term>alpha-Amylases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Gibberellins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches</term><term>Tissu adipeux</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Adipose Tissue</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Differentiation</term><term>Cell Survival</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules souches</term><term>Différenciation cellulaire</term><term>Survie cellulaire</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>alpha-Amylases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adipose Tissue</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Cellules souches</term><term>Tissu adipeux</term><term>alpha-Amylases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Gibbérellines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Gibberellins</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Gibbérellines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Gene Expression</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Expression des gènes</term><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Salivary α-amylase is the most important enzyme for oral digestion of dietary starch. Therefore, regeneration of the salivary glands via a tissue engineering approach is clearly required for patients with salivary gland dysfunction. Early during seed germination, the embryo synthesizes gibberellic acid (GA3), a plant hormone that activates the synthesis and secretion of α-amylase. The purpose of this study was to explore an approach for differentiation of stem cells into salivary glands using GA3. We isolated adipose-derived stem cells (ASCs), which are positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and possess pluripotency to osteoblasts, adipocytes and neural cells, from human buccal fat pads, which are a readily available source for dentists and oral surgeons. In addition, we investigated the cytotoxicity of GA3 for human ASCs. GA3 neither affects cell morphology nor cell viability in a dose- or time-dependent manner. ASCs were incubated with GA3 to assess mRNA and protein expression of α-amylase by reverse transcriptase-polymerase chain reaction and western blot analyses. α-amylase mRNA expression on 21 days after treatment with GA3 (1 mM) was seven times greater than that in resting condition (Day 0). While we did not detect α-amylase bands on Day 0, α-amylase protein was detectable 7 days after treatment with GA3, reaching a maximal level on Day 21. Our results indicated that GA3 can increase cellular α-amylase expression and that our induction method would be useful for therapeutic application for salivary gland regeneration.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22641429</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>19</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1791-244X</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Aug</Month></PubDate></JournalIssue><Title>International journal of molecular medicine</Title><ISOAbbreviation>Int J Mol Med</ISOAbbreviation></Journal><ArticleTitle>Gibberellic acid induces α-amylase expression in adipose-derived stem cells.</ArticleTitle><Pagination><MedlinePgn>243-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3892/ijmm.2012.1007</ELocationID><Abstract><AbstractText>Salivary α-amylase is the most important enzyme for oral digestion of dietary starch. Therefore, regeneration of the salivary glands via a tissue engineering approach is clearly required for patients with salivary gland dysfunction. Early during seed germination, the embryo synthesizes gibberellic acid (GA3), a plant hormone that activates the synthesis and secretion of α-amylase. The purpose of this study was to explore an approach for differentiation of stem cells into salivary glands using GA3. We isolated adipose-derived stem cells (ASCs), which are positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and possess pluripotency to osteoblasts, adipocytes and neural cells, from human buccal fat pads, which are a readily available source for dentists and oral surgeons. In addition, we investigated the cytotoxicity of GA3 for human ASCs. GA3 neither affects cell morphology nor cell viability in a dose- or time-dependent manner. ASCs were incubated with GA3 to assess mRNA and protein expression of α-amylase by reverse transcriptase-polymerase chain reaction and western blot analyses. α-amylase mRNA expression on 21 days after treatment with GA3 (1 mM) was seven times greater than that in resting condition (Day 0). While we did not detect α-amylase bands on Day 0, α-amylase protein was detectable 7 days after treatment with GA3, reaching a maximal level on Day 21. Our results indicated that GA3 can increase cellular α-amylase expression and that our induction method would be useful for therapeutic application for salivary gland regeneration.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kasamatsu</LastName><ForeName>Atsushi</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. kasamatsua@faculty.chiba-u.jp</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Iyoda</LastName><ForeName>Manabu</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Usukura</LastName><ForeName>Katsuya</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Sakamoto</LastName><ForeName>Yosuke</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Ogawara</LastName><ForeName>Katsunori</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Shiiba</LastName><ForeName>Masashi</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tanzawa</LastName><ForeName>Hideki</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Uzawa</LastName><ForeName>Katsuhiro</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>05</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Greece</Country><MedlineTA>Int J Mol Med</MedlineTA><NlmUniqueID>9810955</NlmUniqueID><ISSNLinking>1107-3756</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005875">Gibberellins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>BU0A7MWB6L</RegistryNumber><NameOfSubstance UI="C007842">gibberellic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.1</RegistryNumber><NameOfSubstance UI="D000516">alpha-Amylases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000273" MajorTopicYN="N">Adipose Tissue</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005875" MajorTopicYN="N">Gibberellins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013234" MajorTopicYN="N">Stem Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000516" MajorTopicYN="N">alpha-Amylases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>03</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>04</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22641429</ArticleId><ArticleId IdType="doi">10.3892/ijmm.2012.1007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Iyoda, Manabu" sort="Iyoda, Manabu" uniqKey="Iyoda M" first="Manabu" last="Iyoda">Manabu Iyoda</name><name sortKey="Ogawara, Katsunori" sort="Ogawara, Katsunori" uniqKey="Ogawara K" first="Katsunori" last="Ogawara">Katsunori Ogawara</name><name sortKey="Sakamoto, Yosuke" sort="Sakamoto, Yosuke" uniqKey="Sakamoto Y" first="Yosuke" last="Sakamoto">Yosuke Sakamoto</name><name sortKey="Shiiba, Masashi" sort="Shiiba, Masashi" uniqKey="Shiiba M" first="Masashi" last="Shiiba">Masashi Shiiba</name><name sortKey="Tanzawa, Hideki" sort="Tanzawa, Hideki" uniqKey="Tanzawa H" first="Hideki" last="Tanzawa">Hideki Tanzawa</name><name sortKey="Usukura, Katsuya" sort="Usukura, Katsuya" uniqKey="Usukura K" first="Katsuya" last="Usukura">Katsuya Usukura</name><name sortKey="Uzawa, Katsuhiro" sort="Uzawa, Katsuhiro" uniqKey="Uzawa K" first="Katsuhiro" last="Uzawa">Katsuhiro Uzawa</name></noCountry><country name="Japon"><noRegion><name sortKey="Kasamatsu, Atsushi" sort="Kasamatsu, Atsushi" uniqKey="Kasamatsu A" first="Atsushi" last="Kasamatsu">Atsushi Kasamatsu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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